Young blood plasma is known to confer beneficial effects on various organs in mice. However, it was not known whether young plasma rejuvenates cells and tissues at the epigenetic level; whether it alters the epigenetic clock, which is a highly-accurate molecular biomarker of aging.
Researchers developed and validated six different epigenetic clocks for rat tissues that are based on DNA methylation. They demonstrated that a plasma-derived treatment markedly reverses aging according to epigenetic clocks and benchmark biomarkers of aging. According to the six epigenetic clocks, the plasma fraction treatment rejuvenated liver by 73.4% (ranging from 63% to 81% depending on the clock), blood by 52% (ranging from 47 to 56%), heart by 52% (ranging from 40 to 74%), and hypothalamus by 11% (ranging from 1 to 20%). The rejuvenation effects are even more pronounced if we use the final versions of our epigenetic clocks: liver 75%, blood 66%, heart 57%, hypothalamus 19%. According to the final version of the epigenetic clocks, the average rejuvenation across four tissues was 54.2%.